Article
Variable expression of Cre recombinase transgenes precludes reliable prediction of tissue-specific gene disruption by tail-biopsy genotyping.
PloS one - 10 Oct 2007
Schulz Tim J, Glaubitz Markus, Kuhlow Doreen, Thierbach René, Birringer Marc, Steinberg Pablo, Pfeiffer Andreas F H, Ristow Michael
Abstract excerpt
The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing number of generations. This eventually led to the complete abrogation of gene expression of the inserted Cre cDNA...
Topics
- Animals
- Biopsy
- DNA, Complementary
- Gene Expression Regulation
- Genes, Reporter
- Genetic Techniques
- Genotype
- Integrases
- Mice
- Mice, Transgenic
- Phenotype
- Recombination, Genetic
- Reproducibility of Results