Article
Genotyping of Cre-lox mice and detection of tissue-specific recombination by multiplex PCR.
BioTechniques - 1 Nov 2001
Leneuve P, Zaoui R, Monget P, Le Bouc Y, Holzenberger M
Abstract excerpt
Conditional gene targeting, based on Cre-lox or other systems, requires frequent genotyping of transgenic mouse populations and monitoring of tissue-specific Cre recombinatory efficiency. This is currently achieved by Southern analysis from tail- and tissue-derived DNA. Multiplex PCR amplification of the floxed (flanked by loxP sites) genomic region, combined with the PCR detection of the Cre transgene,...
Topics
- Alleles
- Animals
- Genotype
- Integrases
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Organ Specificity
- Polymerase Chain Reaction
- Recombination, Genetic
- Transgenes
- Viral Proteins