Article
An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues.
Nucleic acids research - 15 May 2003
Scheel John R, Garrett Lisa J, Allen Duane M, Carter Todd A, Randolph-Moore Lynne, Gambello Micheal J, Gage Fred H, Wynshaw-Boris Anthony, Barlow Carrolee
Abstract excerpt
A common method for generating mice with subtle genetic manipulations uses homologous recombination (HR) in embryonic stem (ES) cells to replace a wild-type gene with a slightly modified one. Generally, a drug resistance gene is inserted with the modified gene to select correctly targeted clones. Often, however, the presence of this drug resistance gene interferes with the normal locus and creates a null or...
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