Article
Yeast phenotypic assays on translational control.
Methods in enzymology - 1 Jan 2007
Lee Bumjun, Udagawa Tsuyoshi, Singh Chingakham Ranjit, Asano Katsura
Abstract excerpt
This chapter describes phenotypic assays on specific and general aspects of translation using yeast Saccharomyces cerevisiae as a model eukaryote. To study the effect on start codon selection stringency, a his4(-) or his4-lacZ allele altering the first AUG to AUU is employed. Mutations relaxing the stringent selection confer the His(+) phenotype in the his4(-) strain background or increase expression from...
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