Article
Rapid detection of epidermal growth factor receptor mutations in lung cancer by the SMart-Amplification Process.
Clinical cancer research : an official journal of the American Association for Cancer Research - 1 Sept 2007
Hoshi Kanako, Takakura Hideki, Mitani Yasumasa, Tatsumi Kenji, Momiyama Nobuyoshi, Ichikawa Yasushi, Togo Shinji, Miyagi Toru, Kawai Yuki, Kogo Yasushi, Kikuchi Takeshi, Kato Chiaki, Arakawa Takahiro, Uno Syuji, Cizdziel Paul E, Lezhava Alexander, Ogawa Noburou, Hayashizaki Yoshihide, Shimada Hiroshi
Abstract excerpt
PURPOSE: A positive response to gefitinib in non-small cell lung cancer (NSCLC) has been correlated to mutations in epidermal growth factor receptor (EGFR) gene. Previous reports have been based mainly on diagnostic screening by sequencing. However, sequencing is a time-consuming and complicated procedure, not suitable for routine clinical use. EXPERIMENTAL DESIGN: We have developed rapid, simple, and sensitive...
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