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Article

Mutational analysis of leucine 47 in human epidermal growth factor.

Journal of cellular biochemistry - 1 Jul 1991

Matsunami R K, Yette M L, Stevens A, Niyogi S K

Abstract excerpt

Seven site-specific mutants (including changes to other hydrophobic, charged, and heterocyclic amino acids) of leucine 47 of human epidermal growth factor (EGF) were generated by protein engineering and characterized for their activity in three assays: radioreceptor competition binding in membrane fractions, the stimulation of the EGF receptor's tyrosine kinase activity, and the stimulation of thymidine uptake in...

Topics

Base Sequence
Binding, Competitive
Cell Line
DNA Mutational Analysis
Epidermal Growth Factor
Humans
Leucine
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Mutation
Protein Engineering

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein-Tyrosine Kinases

Radioligand Assay

Thymidine

Source

Identifiers

PMID: 1774224
DOI: 10.1002/jcb.240460307

Genes

EGF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1774224-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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