Article
Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and 1H NMR.
Biochemistry - 10 Sept 1991
Hommel U, Dudgeon T J, Fallon A, Edwards R M, Campbell I D
Abstract excerpt
In order to elucidate the mechanism of interaction between human epidermal growth factor (EGF) and its receptor, selected variants of EGF, differing by single amino acid substitutions, have been made by site-directed mutagenesis. The receptor affinity of these mutants was determined by a receptor...
Topics
- Animals
- Arginine
- Epidermal Growth Factor
- ErbB Receptors
- Genetic Variation
- Haplorhini
- Humans
- Magnetic Resonance Spectroscopy
- Mice
- Mutagenesis, Site-Directed
- Protein Conformation
- Radioligand Assay
- Structure-Activity Relationship
- Tyrosine
- Vero Cells