Structure-function studies of nerve growth factor: functional importance of highly conserved amino acid residues.

doi:10.1002/j.1460-2075.1990.tb08265.x

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Article

Structure-function studies of nerve growth factor: functional importance of highly conserved amino acid residues.

The EMBO journal - 1 May 1990

Ibáñez C F, Hallböök F, Ebendal T, Persson H

Abstract excerpt

Selected amino acid residues in chicken nerve growth factor (NGF) were replaced by site-directed mutagenesis. Mutated NGF sequences were transiently expressed in COS cells and the yield of NGF protein in conditioned medium was quantified by Western blotting. Binding of each mutant to NGF receptor...

Topics

Amino Acid Sequence
Animals
Chickens
Molecular Sequence Data
Mutation
Nerve Growth Factors
Receptor, Insulin
Recombinant Proteins
Sequence Homology, Nucleic Acid
Structure-Activity Relationship

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