Article
Expression and kinetic characterization of variants of human beta 1 beta 1 alcohol dehydrogenase containing substitutions at amino acid 47.
The Journal of biological chemistry - 25 Sept 1990
Hurley T D, Edenberg H J, Bosron W F
Abstract excerpt
Arg-47 of human beta 1 beta 1 alcohol dehydrogenase has been replaced with Lys, His, Gln, and Gly by site-directed mutagenesis. The mutated enzymes were expressed in Escherichia coli and purified to homogeneity. The recombinant enzymes with Arg and His at position 47 exhibit kinetic constants and...
Topics
- Alcohol Dehydrogenase
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Codon
- Computer Graphics
- DNA
- Genetic Variation
- Humans
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Protein Conformation
- Recombinant Proteins