Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evolution of a fluorinated green fluorescent protein.

Proceedings of the National Academy of Sciences of the United States of America - 28 Aug 2007

Yoo Tae Hyeon, Link A James, Tirrell David A

Abstract excerpt

The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20 amino acid substitutions. The mutant protein in fluorinated form showed...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Protein Folding
Share to Solubility
Share to Recombinant Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Protein Denaturation

Share to Amino Acid Substitution

Share to Directed Molecular Evolution

Share to Flow Cytometry

Share to Green Fluorescent Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 17717085
DOI: 10.1073/pnas.0701904104
PMCID: PMC1955812

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evolution of a fluorinated green fluorescent protein.PMID 17717085Read ↗

Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution.Semantic proximity Read ↗

Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids.Semantic proximity Read ↗

Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control.Semantic proximity Read ↗

Engineering a bright near-infrared fluorescent protein by screening a comprehensive phenotypic landscape.Semantic proximity Read ↗

A practical teaching course in directed protein evolution using the green fluorescent protein as a model.Semantic proximity Read ↗

Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria.Semantic proximity Read ↗

Applications of the green fluorescent protein in cell biology and biotechnology.Semantic proximity Read ↗

Modulating protein folding rates in vivo and in vitro by side-chain interactions between the parallel beta strands of green fluorescent protein.Semantic proximity Read ↗

Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position.Semantic proximity Read ↗

Generation of protein lineages with new sequence spaces by functional salvage screen.Semantic proximity Read ↗

Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid.Semantic proximity Read ↗

Engineering green fluorescent protein as a dual functional tag.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.