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Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position.

Protein engineering, design & selection : PEDS - 1 Jun 2005

Kajihara Daisuke, Hohsaka Takahiro, Sisido Masahiko

Abstract excerpt

In order to alter the fluorescence properties of green fluorescent protein (GFP), aromatic non-natural amino acids were introduced into the Tyr66 position of GFP in a cell-free translation system using a four-base codon method. Two non-natural mutants (O-methyltyrosine and p-aminophenylalanine mutants) out of 18 mutants showed blue-shifted but weak fluorescence compared with wild-type GFP. Then the...

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Cell-Free System
Codon
Gene Library
Green Fluorescent Proteins
Phenylalanine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Engineering

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PMID: 15928004
DOI: 10.1093/protein/gzi033

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