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Article

Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control.

PloS one - 11 Jun 2008

Fisher Adam C, DeLisa Matthew P

Abstract excerpt

Green fluorescent protein (GFP) has undergone a long history of optimization to become one of the most popular proteins in all of cell biology. It is thermally and chemically robust and produces a pronounced fluorescent phenotype when expressed in cells of all types. Recently, a superfolder GFP was engineered with increased resistance to denaturation and improved folding kinetics. Here we report that unlike other...

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PMID: 18545653
DOI: 10.1371/journal.pone.0002351
PMCID: PMC2396501

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