Article
Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids.
Molecules (Basel, Switzerland) - 16 Jul 2017
Villa Jordan K, Tran Hong-Anh, Vipani Megha, Gianturco Stephanie, Bhasin Konark, Russell Brent L, Harbron Elizabeth J, Young Douglas D
Abstract excerpt
The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various...
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