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Article

Engineering green fluorescent protein as a dual functional tag.

Biotechnology and bioengineering - 20 Jun 2004

Paramban Rosanto I, Bugos Robert C, Su Wei Wen

Abstract excerpt

A hexa-histidine (6 x His) sequence was inserted into a surface loop of the green fluorescent protein (GFP) to develop a dual functional GFP useful for both monitoring and purification of recombinant proteins. Two variants (GFP172 and GFP157), differentiated by the site of insertion of the 6xHis sequence, were developed and compared with a control variant (GFPHis) having the 6xHis sequence at its C-terminus. The...

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PMID: 15137081
DOI: 10.1002/bit.20077

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MBP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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