Detection of gene targeting by co-conversion of a single nucleotide change during replacement recombination at the immunoglobulin mu heavy chain locus.

Abstract excerpt

A method is described for detecting targeted events at the mu heavy chain gene which relies on co-conversion (or co-exchange) of a point mutation with a selectable marker contained on a replacement vector. The vector, designed for application to IgM producing hybridomas, contains a single nucleotide change within the region of homology with the target gene which encodes a different allotypic determinant of IgM....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Blotting, Southern
• Share to Genetic Markers