Article
Detection of gene targeting by co-conversion of a single nucleotide change during replacement recombination at the immunoglobulin mu heavy chain locus.
Nucleic acids research - 1 Dec 1991
Smith A J, Kalogerakis B
Abstract excerpt
A method is described for detecting targeted events at the mu heavy chain gene which relies on co-conversion (or co-exchange) of a point mutation with a selectable marker contained on a replacement vector. The vector, designed for application to IgM producing hybridomas, contains a single nucleotide change within the region of homology with the target gene which encodes a different allotypic determinant of IgM....
Topics
- Alanine Transaminase
- Animals
- Base Sequence
- Blotting, Southern
- Genes, Immunoglobulin
- Genetic Markers
- Genetic Vectors
- Immunoglobulin mu-Chains
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data