High-frequency loss of specific immunoglobulin production in hybridoma cell lines bearing a chromosomal immunoglobulin kappa gene modified by homologous recombination.

doi:10.1007/BF02290680

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Article

High-frequency loss of specific immunoglobulin production in hybridoma cell lines bearing a chromosomal immunoglobulin kappa gene modified by homologous recombination.

Somatic cell and molecular genetics - 1 Mar 1994

Ng P, Baker M D

Abstract excerpt

We have examined the stability of trinitrophenyl (TNP) -specific IgM production in hybridoma cell lines in which homologous recombination was used to change the variable region of an endogenous chromosomal immunoglobulin kappa gene to one specific for TNP. Mutant hybridomas that have lost TNP-spe...

Topics

Animals
Genes, Immunoglobulin
Hybridomas
Immunoglobulin M
Immunoglobulin kappa-Chains
Mice
Mutation
Recombination, Genetic
Trinitrobenzenes

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