Article
A reporter gene to analyse the hypermutation of immunoglobulin genes.
Journal of molecular biology - 9 Jun 1995
Chui Y L, Lozano F, Jarvis J M, Pannell R, Milstein C
Abstract excerpt
The affinity maturation of antibodies is driven by somatic hypermutation which is localized to specific segments of the coding genes. The information available on this process derives from studies in vivo. With the intention of developing new approaches, we have constructed a fusion gene between...
Topics
- Amino Acid Sequence
- Animals
- B-Lymphocytes
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA Mutational Analysis
- DNA, Recombinant
- Drug Resistance, Microbial
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Genes, Immunoglobulin
- Genes, Reporter
- Mice
- Molecular Sequence Data
- Mutation
- Neomycin
- Polymerase Chain Reaction
- Transfection