Article
Mutation in a reporter gene depends on proximity to and transcription of immunoglobulin variable transgenes.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1991
Umar A, Schweitzer P A, Levy N S, Gearhart J D, Gearhart P J
Abstract excerpt
Somatic mutation in immunoglobulin genes is localized to a 2-kilobase region of DNA surrounding and including rearranged variable (V), diversity, and joining (J) gene segments encoding heavy and light chains. To examine the structural basis for targeted mutation, we developed an assay to score mutation on plasmid substrates by using a reporter gene: a bacterial gene encoding an amber-suppressor tRNA molecule was...
Topics
- Animals
- B-Lymphocytes
- Cell Line
- Cell Nucleus
- Gene Frequency
- Gene Rearrangement
- Genes, Immunoglobulin
- Hybridomas
- Immunoglobulin Joining Region
- Immunoglobulin Variable Region