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Article

Efficient selection of mu m-mutants from mu m-expressing myeloma cells by treatment with ricin A-conjugated anti-mu antibody.

Somatic cell and molecular genetics - 1 Nov 1992

Shindo T, Ueda H, Makishima F, Suzuki E, Nishimura H

Abstract excerpt

We have developed an efficient system for obtaining myeloma mutants defective in trans-acting factors required for immunoglobulin (Ig) gene expression. The system consists of a myeloma cell line designed for this purpose and an efficient method for selecting mutants from it. The cell line is X63.653 transfected with the mu gene, whose tailpiece sequence was replaced with the transmembrane sequence of human EGF...

Topics

Animals
Cell Separation
ErbB Receptors
Immunoglobulin M
Immunotoxins
Mice
Multiple Myeloma
Mutation
Plasmids
Ricin
Trans-Activators
Transfection

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tumor Cells, Cultured

Source

Identifiers

PMID: 1287853
DOI: 10.1007/BF01232651

Genes

EGF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1287853-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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