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Article

Characterization of aberrant melting peaks in unlabeled probe assays.

The Journal of molecular diagnostics : JMD - 1 Jul 2007

Dames Shale, Margraf Rebecca L, Pattison David C, Wittwer Carl T, Voelkerding Karl V

Abstract excerpt

An unlabeled probe assay relies on a double-stranded DNA-binding dye to detect and verify target based on amplicon and probe melting. During the development and application of unlabeled probe assays, aberrant melting peaks are sometimes observed that may interfere with assay interpretation. In this report, we investigated the origin of aberrant melting profiles observed in an unlabeled probe assay for exon 10 of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17591927
DOI: 10.2353/jmoldx.2007.060139
PMCID: PMC1899421

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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