Article
Unlabeled probes for the detection and typing of herpes simplex virus.
Clinical chemistry - 1 Oct 2007
Dames Shale, Pattison David C, Bromley L Kathryn, Wittwer Carl T, Voelkerding Karl V
Abstract excerpt
BACKGROUND: Unlabeled probe detection with a double-stranded DNA (dsDNA) binding dye is one method to detect and confirm target amplification after PCR. Unlabeled probes and amplicon melting have been used to detect small deletions and single-nucleotide polymorphisms in assays where template is in abundance. Unlabeled probes have not been applied to low-level target detection, however. METHODS: Herpes simplex...
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