Article
Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting.
American journal of clinical pathology - 1 Mar 2007
Liew Michael, Seipp Michael, Durtschi Jacob, Margraf Rebecca L, Dames Shale, Erali Maria, Voelkerding Karl, Wittwer Carl
Abstract excerpt
Two methods for closed-tube single nucleotide polymorphism (SNP) genotyping without labeled probes have become available: unlabeled probe and amplicon melting. Unlabeled probe and amplicon melting assays were compared using 5 SNPs: human platelet antigens 1, 2, 5, and 15 and a C>T variant located 13910 base pairs (bp) upstream of the lactase gene. LCGreen Plus (Idaho Technology, Salt Lake City, UT) was used as...
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