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Article

Masking selected sequence variation by incorporating mismatches into melting analysis probes.

Human mutation - 1 Mar 2006

Margraf Rebecca L, Mao Rong, Wittwer Carl T

Abstract excerpt

Hybridization probe melting analysis can be complicated by the presence of sequence variation (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of "masking" probes to differentiate alleles with similar probe melting temperatures. Selected sequence variation was masked by incorporating mismatches (deletion, unmatched nucleotide, or universal base) into hybridization...

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Share to Base Pair Mismatch
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Nucleic Acid Hybridization

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Share to Proto-Oncogene Proteins c-ret

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PMID: 16411177
DOI: 10.1002/humu.20290

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RET

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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