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Article

RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis.

The Journal of molecular diagnostics : JMD - 1 Apr 2007

Margraf Rebecca L, Mao Rong, Highsmith W Edward, Holtegaard Leonard M, Wittwer Carl T

Abstract excerpt

Single bp mutations in the RET proto-oncogene can cause multiple endocrine neoplasia type 2 syndromes. The conventional approach for genotyping RET mutations is sequencing the exons. A closed-tube RET genotyping assay using a saturating DNA dye, unlabeled probes, and amplicon high-resolution melting analysis was developed. The method required two sequential polymerase chain reaction stages, a primary and...

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Identifiers

PMID: 17384210
DOI: 10.2353/jmoldx.2007.060091
PMCID: PMC1867443

Genes

RET

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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