Article
Functional expression of the Flp recombinase in Mycobacterium bovis BCG.
Gene - 15 Sept 2007
Song Houhui, Niederweis Michael
Abstract excerpt
Mycobacteria contain a large number of redundant genes whose functions are difficult to analyze in mutants because there are only two efficient antibiotic resistance genes available for allelic exchange experiments. Sequence-specific recombinbases such as the Flp recombinase can be used to excise resistance markers. Expression of the flp(e) gene from Saccharomyces cerevisiae is functional for this purpose in...
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