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Efficient construction of unmarked recombinant mycobacteria using an improved system.

Journal of microbiological methods - 1 Aug 2014

Yang Feng, Tan Yaoju, Liu Jia, Liu Tianzhou, Wang Bangxing, Cao Yuanyuan, Qu Yue, Lithgow Trevor, Tan Shouyong, Zhang Tianyu

Abstract excerpt

The genetic study of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium ulcerans, is hampered heavily by their slow growth. We have developed efficient, versatile, and improved genetic tools for constructing unmarked recombinant mycobacteria more rapidly including generating multiple mutants using the same antibiotic marker in both fast- and slow-growing mycobacteria.

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Identifiers

PMID: 24873745
DOI: 10.1016/j.mimet.2014.05.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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