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Article

Production of mutants in amino acid biosynthesis genes of Mycobacterium tuberculosis by homologous recombination.

Microbiology (Reading, England) - 1 Dec 1999

Parish Tanya, Gordhan Bhavna G, McAdam Ruth A, Duncan Ken, Mizrahi Valerie, Stoker Neil G

Abstract excerpt

The ability to generate mutants of Mycobacterium tuberculosis will be important if we are to understand the biology of this major pathogen. However, allelic replacement methods have only recently achieved success. We have developed a reproducible method for generating defined mutants of M. tuberculosis using homologous recombination. The transforming DNA was used following pre-treatment either with UV light or...

Topics

Amino Acids
Crossing Over, Genetic
DNA, Bacterial
Electroporation
Genetic Vectors
Hydrogen-Ion Concentration
Lac Operon
Mutation
Mycobacterium tuberculosis
Plasmids
Recombination, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transformation, Bacterial

Ultraviolet Rays

Source

Identifiers

PMID: 10627047
DOI: 10.1099/00221287-145-12-3497

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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