Article
Mycobacterial recombineering.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Murphy Kenan C, Papavinasasundaram Kadamba, Sassetti Christopher M
Abstract excerpt
The precise knockout or modification of Mycobacterium tuberculosis genes has been critical for the identification of functions important for the growth and pathogenicity of this important bacterium. Schemes have been previously described, using both non-replicating vectors and transducing particles, for the introduction of gene knockout substrates into M. tuberculosis, where the endogenous recombination systems...
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