Article
Insertional mutagenesis and illegitimate recombination in mycobacteria.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1991
Kalpana G V, Bloom B R, Jacobs W R
Abstract excerpt
Mycobacteria, particularly Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium avium, are major pathogens of man. Although insertional mutagenesis has been an invaluable genetic tool for analyzing the mechanisms of microbial pathogenesis, it has not yet been possible to apply it to the mycobacteria. To overcome intrinsic difficulties in directly manipulating the genetics of slow-growing...
Topics
- Blotting, Southern
- DNA, Circular
- Genes, Bacterial
- Mutagenesis
- Mutation
- Mycobacterium
- Plasmids
- Recombination, Genetic