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Article

The use of temperature-sensitive plasmids in mycobacteria.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Portevin Damien, Malaga Wladimir, Guilhot Christophe

Abstract excerpt

The construction of allelic exchange mutants is one of the major strategies to decipher the function of a defined gene. In this chapter, protocols are described to perform allelic exchange in the mycobacterial species Mycobacterium smegmatis and Mycobacterium tuberculosis using thermosensitive counterselectable vectors. The antibiotic resistance cassette used makes it possible to efficiently rescue the marker,...

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Source

Identifiers

PMID: 20560074
DOI: 10.1007/978-1-59745-207-6_15

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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