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Article

Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis.

Microbiology (Reading, England) - 1 Oct 2002

Bardarov Stoyan, Bardarov Svetoslav, Pavelka Martin S, Sambandamurthy Vasan, Larsen Michelle, Tufariello JoAnn, Chan John, Hatfull Graham, Jacobs William R

Abstract excerpt

The authors have developed a simple and highly efficient system for generating allelic exchanges in both fast- and slow-growing mycobacteria. In this procedure a gene of interest, disrupted by a selectable marker, is cloned into a conditionally replicating (temperature-sensitive) shuttle phasmid to generate a specialized transducing mycobacteriophage. The temperature-sensitive mutations in the mycobacteriophage...

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PMID: 12368434
DOI: 10.1099/00221287-148-10-3007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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