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Article

Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase.

Gene - 8 Dec 2004

Stephan Joachim, Stemmer Volker, Niederweis Michael

Abstract excerpt

Mycobacteria contain a large number of redundant genes whose functions are difficult to analyze in mutants, because there are only two efficient resistance markers available for allelic exchange experiments. We have established a system based on the Flp recombinase of the yeast Saccharomyces cerevisiae for use in the nonpathogenic model organism Mycobacterium smegmatis. This system consists of a hygromycin...

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Identifiers

PMID: 15563844
DOI: 10.1016/j.gene.2004.08.028

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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