Article
Mycobacterial recombineering.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
van Kessel Julia C, Hatfull Graham F
Abstract excerpt
Although substantial advances have been made in mycobacterial genetics over the past 15 yr, manipulation of mycobacterial genomes and Mycobacterium tuberculosis in particular, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination. In Escherichia coli an effective approach to stimulating recombination frequencies has been developed...
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