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Gene replacement and expression of foreign DNA in mycobacteria

1 Feb 1990

Abstract excerpt

A system that permits molecular genetic manipulation of mycobacteria was developed on the basis of the yeast paradigm of gene replacement by homologous recombination. A shuttle vector that can replicate autonomously at a high copy number in Escherichia coli but must integrate into homologous DNA for survival in Mycobacterium smegmatis was constructed. The vector contains a ColE1 origin of replication, antibiotic...

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Identifiers

PMID: 2153655
DOI: 10.1128/jb.172.2.519-524.1990

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