Article
Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity.
Nucleic acids research - 1 Jan 2007
Urbonavicius Jaunius, Jäger Gunilla, Björk Glenn R
Abstract excerpt
The Escherichia coli trmA gene encodes the tRNA(m5U54)methyltransferase, which catalyses the formation of m5U54 in tRNA. During the synthesis of m5U54, a covalent 62-kDa TrmA-tRNA intermediate is formed between the amino acid C324 of the enzyme and the 6-carbon of uracil. We have analysed the formation of this TrmA-tRNA intermediate and m5U54 in vivo, using mutants with altered TrmA. We show that the amino acids...
Topics
- Amino Acid Sequence
- Catalysis
- Enzyme Stability
- Escherichia coli
- Escherichia coli Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- RNA, Transfer
- Sequence Alignment
- Thymine
- tRNA Methyltransferases