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Article

Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations.

Analytical biochemistry - 15 Dec 2015

Botezatu Irina V, Nechaeva Irina O, Stroganova Аnna М, Senderovich Anastasia I, Kondratova Valentina N, Shelepov Valery P, Lichtenstein Anatoly V

Abstract excerpt

The TaqMan probes that have been long and effectively used in real-time polymerase chain reaction (PCR) may also be used in DNA melting analysis. We studied some factors affecting efficiency of the approach such as (i) number of asymmetric PCR cycles preceding DNA melting analysis, (ii) choice of fluorophores for the multiplex DNA melting analysis, and (iii) choice of sense or antisense TaqMan probes for optimal...

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Identifiers

PMID: 26384642
DOI: 10.1016/j.ab.2015.09.005

Genes

KRAS
BRAF
NRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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