Article
[Antigenicity of major hydrophilic region II of hepatitis B virus surface antigen].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Feb 2007
Tian Yong-jun, Zhang Zheng-mao, Xia Chang, Liu Shen-pei, Yu Yuan, Huang Hong-ping, Yang Yan, Lu Meng-ji, Yang Dong-liang
Abstract excerpt
OBJECTIVE: To study whether the substitutions at the major hydrophilic region II (MHRII) of hepatitis B surface antigen (HBsAg) will impair the antigenicity of HBsAg. METHODS: Four recombinant plasmids expressing mutant HBsAg (mtHBsAg) P1120T, C121S, K122I and T123N were constructed. HepG2 cells were transfected with the four plasmids and a plasmid expressing G145R HBsAg. The immunoreactivity of the cells...
Topics
- Amino Acid Substitution
- Antigenic Variation
- Hep G2 Cells
- Hepatitis B Surface Antigens
- Hepatitis B virus
- Humans
- Mutation
- Plasmids
- Transfection