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Article

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons.

Proceedings of the National Academy of Sciences of the United States of America - 27 Feb 2007

Ambrogelly Alexandre, Gundllapalli Sarath, Herring Stephanie, Polycarpo Carla, Frauer Carina, Söll Dieter

Abstract excerpt

Pyrrolysine (Pyl), the 22nd naturally encoded amino acid, gets acylated to its distinctive UAG suppressor tRNA(Pyl) by the cognate pyrrolysyl-tRNA synthetase (PylRS). Here we determine the RNA elements required for recognition and aminoacylation of tRNA(Pyl) in vivo by using the Pyl analog N-epsilon-cyclopentyloxycarbonyl-l-lysine. Forty-two Methanosarcina barkeri tRNA(Pyl) variants were tested in Escherichia...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17360621
DOI: 10.1073/pnas.0611634104
PMCID: PMC1805618

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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