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Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion.

ACS synthetic biology - 16 Oct 2020

Hu Liming, Qin Xuewen, Huang Yujia, Cao Wenbing, Wang Chuchen, Wang Yong, Ling Xinyu, Chen Heqi, Wu Dan, Lin Yu, Liu Tao

Abstract excerpt

Genetic code expansion (GCE) is a powerful technique for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells, which is achieved through evolved aminoacyl-tRNA synthetase mutants. Stability is important for promoting enzyme evolution, and we found that many of the evolved synthetase mutants have reduced thermostabilities. In this study, we characterized two novel...

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PMID: 32931698
DOI: 10.1021/acssynbio.0c00257

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