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Article

Misacylation of pyrrolysine tRNA in vitro and in vivo.

FEBS letters - 15 Oct 2008

Gundllapalli Sarath, Ambrogelly Alexandre, Umehara Takuya, Li Darrick, Polycarpo Carla, Söll Dieter

Abstract excerpt

Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacterial-type seryl-tRNA synthetase in vitro and in vivo in Escherichia coli. Compared to the M. barkeri...

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Identifiers

PMID: 18775710
DOI: 10.1016/j.febslet.2008.08.027
PMCID: PMC2577721

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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