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Article

Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency.

Amino acids - 1 Jan 2021

Williams Thomas L, Iskandar Debra J, Nödling Alexander R, Tan Yurong, Luk Louis Y P, Tsai Yu-Hsuan

Abstract excerpt

Genetic code expansion is a powerful technique for site-specific incorporation of an unnatural amino acid into a protein of interest. This technique relies on an orthogonal aminoacyl-tRNA synthetase/tRNA pair and has enabled incorporation of over 100 different unnatural amino acids into ribosomally synthesized proteins in cells. Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA from Methanosarcina species...

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Identifiers

PMID: 33331978
DOI: 10.1007/s00726-020-02927-z
PMCID: PMC7822784

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