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<i>Methanomethylophilus alvus Mx1201</i> provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells

2018-07-19

Abstract excerpt

<h4>ABSTRACT</h4> Genetic code expansion via stop codon suppression is a powerful technique for engineering proteins in mammalian cells with site-specifically encoded non-canonical amino acids (ncAAs). Current methods rely on very few available tRNA/aminoacyl-tRNA synthetase pairs orthogonal in mammalian cells, the pyrrolysyl tRNA/aminoacyl-tRNA synthetase pair from Methanosarcina mazei ( Mma PylRS/PylT) being...

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Literature Corpus work: 2e0f5e3c-47a6-5fd0-b8e3-6d1e81b21ec0
DOI: 10.1101/371757

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