Article
Analysis of the fibroblast transformation potential of GTPase-deficient gip2 oncogenes.
Molecular and cellular biology - 1 Jan 1992
Gupta S K, Gallego C, Lowndes J M, Pleiman C M, Sable C, Eisfelder B J, Johnson G L
Abstract excerpt
Expression of GTPase-deficient Gi2 alpha subunit (alpha i2) mutant polypeptides and overexpression of the wild-type alpha i2 polypeptide in Rat 1a, Swiss 3T3, and NIH 3T3 fibroblasts altered normal growth regulation and induced a loss of contact inhibition. In Rat 1a cells (but not in NIH 3T3 or Swiss 3T3 cells), expression of the GTPase-deficient alpha i2 mutant polypeptides allowed colony formation in soft...
Topics
- 3T3 Cells
- Animals
- Antibodies
- Cell Division
- Cell Line
- Cell Transformation, Neoplastic
- Fibroblasts
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Gene Expression Regulation, Enzymologic
- Mice