Article
Crystallization and preliminary X-ray crystallographic analysis of the receptor-uncoupled mutant of Galphai1.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Feb 2007
Morikawa Tomohito, Muroya Ayumu, Nakajima Yoshitaka, Tanaka Takeshi, Hirai Keiko, Sugio Shigetoshi, Wakamatsu Kaori, Kohno Toshiyuki
Abstract excerpt
In order to understand the molecular mechanisms by which G-protein-coupled receptors (GPCRs) activate G proteins, the K349P mutant of Galpha(i1) (K349P), which is unable to couple to the muscarinic acetylcholine receptor, was prepared and its crystals were grown along with those of wild-type Galpha(i1) protein (WT). The two proteins were crystallized under almost identical conditions, thus enabling a detailed...
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