Article
Crystallization and preliminary crystallographic studies of Gi alpha 1 and mutants of Gi alpha 1 in the GTP and GDP-bound states.
Journal of molecular biology - 13 May 1994
Coleman D E, Lee E, Mixon M B, Linder M E, Berghuis A M, Gilman A G, Sprang S R
Abstract excerpt
Several different crystal forms of Gi alpha 1 have been grown and analyzed. Crystals of native protein containing bound GTP gamma S belong to space group P3(1)2(1) or P3(2)2(1) with cell dimensions a,b = 80.6 A and c = 106.3 A and diffract to a resolution of 1.9 A using synchrotron radiation. Cry...
Topics
- Crystallization
- Crystallography, X-Ray
- GTP-Binding Proteins
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Diphosphate
- Guanosine Triphosphate
- Mutation
- Protein Binding
- Recombinant Proteins