Article
Mutation screening of bleomycin-induced V79 Chinese hamster hprt mutants using multiplex polymerase chain reaction.
Mutagenesis - 1 Nov 1991
Köberle B, Haupter S, Just W, Speit G
Abstract excerpt
We have shown by the filter hybridization technique that bleomycin (BLM) induces different types of mutations at the hprt gene locus of V79 Chinese hamster cells. DNA of mutants identified by Southern blots as partial deletions was subjected to further analysis using multiplex polymerase chain reaction (PCR) to localize the endpoints of the deletions over the hprt gene. The PCR analysis revealed that deletions...
Topics
- Animals
- Bleomycin
- Blotting, Southern
- Cells, Cultured
- Chromosome Deletion
- Cricetinae
- Cricetulus
- DNA
- DNA Mutational Analysis
- Exons
- Hypoxanthine Phosphoribosyltransferase