Article
A newly established GDL1 cell line from gpt delta mice well reflects the in vivo mutation spectra induced by mitomycin C.
Mutation research - 10 Oct 2006
Takeiri Akira, Mishima Masayuki, Tanaka Kenji, Shioda Akifumi, Harada Asako, Watanabe Kazuto, Masumura Ken-Ichi, Nohmi Takehiko
Abstract excerpt
In order to create a novel in vitro test system for detection of large deletions and point mutations, we developed an immortalized cell line. A SV40 large T antigen expression unit was introduced into fibroblasts derived from gpt delta mouse lung tissue and a selected clone was established as the gpt delta L1 (GDL1) cell line. The novel GDL1 cells were examined for mutant frequencies (MFs) and for molecular...
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