Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Shuttle mutagenesis and targeted disruption of a telomere-located essential gene of Leishmania.

Parasitology - 1 Apr 2007

Squina F M, Pedrosa A L, Nunes V S, Cruz A K, Tosi L R O

Abstract excerpt

Leishmania mutants have contributed greatly to extend our knowledge of this parasite's biology. Here we report the use of the mariner in vitro transposition system as a source of reagents for shuttle mutagenesis and targeted disruption of Leishmania genes. The locus-specific integration was achieved by the disruption of the subtelomeric gene encoding a DNA-directed RNA polymerase III subunit (RPC2). Further...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line
Share to Gene Expression Regulation
Share to Genes, Essential

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Leishmania major

Share to Mutagenesis

Share to Protozoan Proteins

Share to RNA Polymerase III

Share to Telomere

Source

Identifiers

PMID: 17169165
DOI: 10.1017/S0031182006001892

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Shuttle mutagenesis and targeted disruption of a telomere-located essential gene of Leishmania.PMID 17169165Read ↗

Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasiteSemantic proximity Read ↗

DNA Integration in Leishmania Genome: An Application for Vaccine Development and Drug Screening.Semantic proximity Read ↗

The effect of location and direction of an episomal gene on the restoration of a phenotype by functional complementation in Leishmania.Semantic proximity Read ↗

Trans-kingdom Transposition of the <i>Drosophila</i> Element <i>mariner</i> Within the Protozoan <i>Leishmania</i>Semantic proximity Read ↗

Spontaneous excision and facilitated recovery as a control for phenotypes arising from RNA interference and other dominant transgenes.Semantic proximity Read ↗

Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania.Semantic proximity Read ↗

Double targeted gene replacement for creating null mutants.Semantic proximity Read ↗

Replacement of Leishmania N-acetylglucosamine-1-phosphate transferase gene requires episomal rescue.Semantic proximity Read ↗

Application of CRISPR/Cas9-Based Reverse Genetics in Leishmania braziliensis: Conserved Roles for HSP100 and HSP23.Semantic proximity Read ↗

Mapping of a Leishmania major gene/locus that confers pentamidine resistance by deletion and insertion of transposable element.Semantic proximity Read ↗

Gene disruptions indicate an essential function for the LmmCRK1 cdc2-related kinase of Leishmania mexicana.Semantic proximity Read ↗

Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.