Article
Spontaneous excision and facilitated recovery as a control for phenotypes arising from RNA interference and other dominant transgenes.
Molecular and biochemical parasitology - 1 Mar 2018
Brettmann Erin A, Lye Lon-Fye, Beverley Stephen M
Abstract excerpt
An essential control for genetic manipulation of microbes is the regeneration of the wild-type state and phenotype to validate that any mutant phenotypes are 'on target'. For Leishmania gene knockouts, this is often done by re-expression of the target gene from episomal vectors, often bearing counter-selectable markers. Methods for similarly validating the outcomes from dominant mutations such as those arising...
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