Article
Gene disruptions indicate an essential function for the LmmCRK1 cdc2-related kinase of Leishmania mexicana.
Molecular microbiology - 1 Nov 1996
Mottram J C, McCready B P, Brown K G, Grant K M
Abstract excerpt
The generation of homozygous null mutants for the crk1 Cdc2-Related Kinase of Leishmania mexicana was attempted using targeted gene disruption. Promastigote mutants heterozygous for crk1 were readily isolated with a hyg-targeting fragment, but attempts to create null mutants by second-round trans...
Topics
- Alleles
- Animals
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Caseins
- Chromosome Mapping
- DNA, Protozoan
- Gene Expression Regulation
- Genetic Complementation Test
- Histidine
- Leishmania mexicana
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nitrilotriacetic Acid
- Organometallic Compounds
- Plasmids
- Ploidies